13099 1 ap Search Results


twist1  (Proteintech)
96
Proteintech twist1
Twist1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/twist1/product/Proteintech
Average 96 stars, based on 1 article reviews
twist1 - by Bioz Stars, 2026-03
96/100 stars
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anti-snail1 (cat. no. 13099-1-ap)  (Wuhan Sanying Biotechnology)
90
Wuhan Sanying Biotechnology anti-snail1 (cat. no. 13099-1-ap)
Depletion of NET inhibits Notch1 signaling in human colon cancer cells. HCT116 and SW480 cells were treated with NET-targeting siRNAs (siNET1 and siNET2) or negative control siRNA (siNC). After 48 h, cell lysates were harvested, and the protein samples were separated by SDS-PAGE. The levels of full length Notch1, cleaved Notch1 and <t>Snail1</t> were detected by western blotting, and GAPDH was used as the loading control. The band intensities of NET relative to GAPDH were quantified and normalized to the siNC sample. The data are representative of three independent experiments. One-way ANOVA was used to compare data between siNET- and siNC-transfected cells. The least significance difference test was used as the post hoc test to conduct multiple comparisons. *P<0.05, **P<0.01. NET, norepinephrine transporter; siRNA, small interfering RNA.
Anti Snail1 (Cat. No. 13099 1 Ap), supplied by Wuhan Sanying Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-snail1 (cat. no. 13099-1-ap)/product/Wuhan Sanying Biotechnology
Average 90 stars, based on 1 article reviews
anti-snail1 (cat. no. 13099-1-ap) - by Bioz Stars, 2026-03
90/100 stars
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Depletion of NET inhibits Notch1 signaling in human colon cancer cells. HCT116 and SW480 cells were treated with NET-targeting siRNAs (siNET1 and siNET2) or negative control siRNA (siNC). After 48 h, cell lysates were harvested, and the protein samples were separated by SDS-PAGE. The levels of full length Notch1, cleaved Notch1 and Snail1 were detected by western blotting, and GAPDH was used as the loading control. The band intensities of NET relative to GAPDH were quantified and normalized to the siNC sample. The data are representative of three independent experiments. One-way ANOVA was used to compare data between siNET- and siNC-transfected cells. The least significance difference test was used as the post hoc test to conduct multiple comparisons. *P<0.05, **P<0.01. NET, norepinephrine transporter; siRNA, small interfering RNA.

Journal: Oncology Letters

Article Title: Norepinephrine transporter promotes the invasion of human colon cancer cells

doi: 10.3892/ol.2019.11146

Figure Lengend Snippet: Depletion of NET inhibits Notch1 signaling in human colon cancer cells. HCT116 and SW480 cells were treated with NET-targeting siRNAs (siNET1 and siNET2) or negative control siRNA (siNC). After 48 h, cell lysates were harvested, and the protein samples were separated by SDS-PAGE. The levels of full length Notch1, cleaved Notch1 and Snail1 were detected by western blotting, and GAPDH was used as the loading control. The band intensities of NET relative to GAPDH were quantified and normalized to the siNC sample. The data are representative of three independent experiments. One-way ANOVA was used to compare data between siNET- and siNC-transfected cells. The least significance difference test was used as the post hoc test to conduct multiple comparisons. *P<0.05, **P<0.01. NET, norepinephrine transporter; siRNA, small interfering RNA.

Article Snippet: Anti-E-cadherin (cat. no. 20874-1-AP), anti-N-cadherin (cat. no. 22018-1-AP), anti-Notch1 (cat. no. 20687-1-AP) and anti-Snail1 (cat. no. 13099-1-AP) were purchased from Wuhan Sanying Biotechnology, while anti-GAPDH (cat. co. Sc-32233) was obtained from Santa Cruz Biotechnology, Inc.

Techniques: Negative Control, SDS Page, Western Blot, Transfection, Small Interfering RNA